Anticancer activity of Pupalia lappacea on chronic myeloid leukemia K562 cells.

ABSTRACT:

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Background

Cancer is one of the most prominent human diseases which has enthused scientific and commercial interest in the discovery of newer anticancer agents from natural sources. Here we demonstrated the anticancer activity of ethanolic extract of aerial parts of Pupalia lappacea (L) Juss (Amaranthaceae) (EAPL) on Chronic Myeloid Leukemia K562 cells.

Methods

Antiproliferative activity of EAPL was determined by MTT assay using carvacrol as a positive control. Induction of apoptosis was studied by annexin V, mitochondrial membrane potential, caspase activation and cell cycle analysis using flow cytometer and modulation in protein levels of p53, PCNA, Bax and Bcl2 ratio, cytochrome c and cleavage of PARP were studied by Western blot analysis. The standardi

SUBMITTER: Ravi A 

PROVIDER: S-EPMC3559012 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature