RACK1 is involved in endothelial barrier regulation via its two novel interacting partners.

ABSTRACT:

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Background

RACK1, receptor for activated protein kinase C, serves as an anchor in multiple signaling pathways. TIMAP, TGF-β inhibited membrane-associated protein, is most abundant in endothelial cells with a regulatory effect on the endothelial barrier function. The interaction of TIMAP with protein phosphatase 1 (PP1cδ) was characterized, yet little is known about its further partners.

Results

We identified two novel interacting partners of RACK1, namely, TGF-β inhibited membrane-associated protein, TIMAP, and farnesyl transferase. TIMAP is most abundant in endothelial cells where it is involved in the regulation of the barrier function. WD1-4 repeats of RACK1 were identified as critical regions of the interaction both with TIMAP and farnesyl transferase.

SUBMITTER: Boratko A 

PROVIDER: S-EPMC3560227 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature