Skelemin in integrin α(IIb)β(3) mediated cell spreading.

ABSTRACT: Skelemin, a myosin-associated protein in skeletal muscle, has been demonstrated to interact with integrin α(IIb)β(3) in nonmuscle cells during initial stages of cell spreading. The significance of this interaction and the role of skelemin in integrin signaling and cytoskeletal reorganization were investigated in this study. We established a series of Chinese hamster ovary cell lines expressing wild-type or mutant α(IIb)β(3) receptors in which skelemin binding residues at the membrane proximal region of integrin tails were mutated to alanine. Most cells displayed unimpaired adhesive capacity and spreading on immobilized fibrinogen at the early stages of cell spreading. In addition, they formed normal focal adhesions and stress fibers with no indication of impaired cell spreading. R995A/R997

SUBMITTER: Li X 

PROVIDER: S-EPMC3560429 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature