Increased expression of DNA repair gene XPF enhances resistance to hydroxycamptothecin in bladder cancer.

ABSTRACT:

Background

Xeroderma pigmentosum group F (XPF) is an important participant in the nucleotide excision repair process. This study aimed to investigate the expression of DNA repair gene xeroderma pigmentosum group F (XPF) in bladder cancer and its clinical significance.

Material/methods

Total RNA and protein were extracted from 45 untreated bladder cancer tissues and 21 hydroxycamptothecin (HCPT)-treated bladder cancer specimens. Real-time PCR and Western blot assay were used to detect the mRNA and protein levels of XPF, respectively. siRNA targeting XPF was used to knock down the XPF expression in T24 cells and 5637 cells, and the sensitivity of XPF-depleted cells to HCPT was measured.

Results

The XPF expressions in the HCPT-treated cancer tissues was significantly hig

SUBMITTER: Li J 

PROVIDER: S-EPMC3560829 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature