Construction of the first genetic linkage map of Japanese gentian (Gentianaceae).

ABSTRACT:

Background

Japanese gentians (Gentiana triflora and Gentiana scabra) are amongst the most popular floricultural plants in Japan. However, genomic resources for Japanese gentians have not yet been developed, mainly because of the heterozygous genome structure conserved by outcrossing, the long juvenile period, and limited knowledge about the inheritance of important traits. In this study, we developed a genetic linkage map to improve breeding programs of Japanese gentians.

Results

Enriched simple sequence repeat (SSR) libraries from a G. triflora double haploid line yielded almost 20,000 clones using 454 pyrosequencing technology, 6.7% of which could be used to design SSR markers. To increase the number of molecular markers, we identified three putative long terminal repeat (

SUBMITTER: Nakatsuka T 

PROVIDER: S-EPMC3561071 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature