Cell population-specific expression analysis of human cerebellum.

ABSTRACT:

Background

Interpreting gene expression profiles obtained from heterogeneous samples can be difficult because bulk gene expression measures are not resolved to individual cell populations. We have recently devised Population-Specific Expression Analysis (PSEA), a statistical method that identifies individual cell types expressing genes of interest and achieves quantitative estimates of cell type-specific expression levels. This procedure makes use of marker gene expression and circumvents the need for additional experimental information like tissue composition.

Results

To systematically assess the performance of statistical deconvolution, we applied PSEA to gene expression profiles from cerebellum tissue samples and compared with parallel, experimental separation methods. Ow

SUBMITTER: Kuhn A 

PROVIDER: S-EPMC3561119 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature