Aberrant methylation of the M-type phospholipase A(2) receptor gene in leukemic cells.

ABSTRACT:

Background

The M-type phospholipase A2 receptor (PLA2R1) plays a crucial role in several signaling pathways and may act as tumor-suppressor. This study examined the expression and methylation of the PLA2R1 gene in Jurkat and U937 leukemic cell lines and its methylation in patients with myelodysplastic syndrome (MDS) or acute leukemia.

Methods

Sites of methylation of the PLA2R1 locus were identified by sequencing bisulfite-modified DNA fragments. Methylation specific-high resolution melting (MS-HRM) analysis was then carried out to quantify PLA2R1 methylation at 5'-CpG sites identified with differences in methylation between healthy control subjects and leukemic patients using sequencing of bisulfite-modified genomic DNA.

Results

Expression of PLA2R1 was found to be co

SUBMITTER: Menschikowski M 

PROVIDER: S-EPMC3561142 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature