Class II HDAC inhibition hampers hepatic stellate cell activation by induction of microRNA-29.

ABSTRACT:

Background

The conversion of a quiescent vitamin A storing hepatic stellate cell (HSC) to a matrix producing, contractile myofibroblast-like activated HSC is a key event in the onset of liver disease following injury of any aetiology. Previous studies have shown that class I histone deacetylases (HDACs) are involved in the phenotypical changes occurring during stellate cell activation in liver and pancreas.

Aims

In the current study we investigate the role of class II HDACs during HSC activation.

Methods

We characterized the expression of the class II HDACs freshly isolated mouse HSCs. We inhibited HDAC activity by selective pharmacological inhibition with MC1568, and by repressing class II HDAC gene expression using specific siRNAs.

Results

Inhibition of HDAC

SUBMITTER: Mannaerts I 

PROVIDER: S-EPMC3561334 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature