Combining functional and structural genomics to sample the essential Burkholderia structome.

ABSTRACT:

Background

The genus Burkholderia includes pathogenic gram-negative bacteria that cause melioidosis, glanders, and pulmonary infections of patients with cancer and cystic fibrosis. Drug resistance has made development of new antimicrobials critical. Many approaches to discovering new antimicrobials, such as structure-based drug design and whole cell phenotypic screens followed by lead refinement, require high-resolution structures of proteins essential to the parasite.

Methodology/principal findings

We experimentally identified 406 putative essential genes in B. thailandensis, a low-virulence species phylogenetically similar to B. pseudomallei, the causative agent of melioidosis, using saturation-level transposon mutagenesis and next-generation sequencing (Tn-seq). We select

SUBMITTER: Baugh L 

PROVIDER: S-EPMC3561365 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature