In vivo monitoring of cardiomyocyte proliferation to identify chemical modifiers of heart regeneration.

ABSTRACT: Adult mammalian cardiomyocytes have little capacity to proliferate in response to injury, a deficiency that underlies the poor regenerative ability of human hearts after myocardial infarction. By contrast, zebrafish regenerate heart muscle after trauma by inducing proliferation of spared cardiomyocytes, providing a model for identifying manipulations that block or enhance these events. Although direct genetic or chemical screens of heart regeneration in adult zebrafish present several challenges, zebrafish embryos are ideal for high-throughput screening. Here, to visualize cardiomyocyte proliferation events in live zebrafish embryos, we generated transgenic zebrafish lines that employ fluorescent ubiquitylation-based cell cycle indicator (FUCCI) technology. We then performed a chemical scr

SUBMITTER: Choi WY 

PROVIDER: S-EPMC3561784 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature