Identification of proteins binding coding and non-coding human RNAs using protein microarrays.

ABSTRACT:

Background

The regulation and function of mammalian RNAs has been increasingly appreciated to operate via RNA-protein interactions. With the recent discovery of thousands of novel human RNA molecules by high-throughput RNA sequencing, efficient methods to uncover RNA-protein interactions are urgently required. Existing methods to study proteins associated with a given RNA are laborious and require substantial amounts of cell-derived starting material. To overcome these limitations, we have developed a rapid and large-scale approach to characterize binding of in vitro transcribed labeled RNA to ~9,400 human recombinant proteins spotted on protein microarrays.

Results

We have optimized methodology to probe human protein microarrays with full-length RNA molecules and have ident

SUBMITTER: Siprashvili Z 

PROVIDER: S-EPMC3562209 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature