Neutrophil proteinase 3 acts on protease-activated receptor-2 to enhance vascular endothelial cell barrier function.

ABSTRACT:

Objective

The principle role of the vascular endothelium is to present a semi-impermeable barrier to soluble factors and circulating cells, while still permitting the passage of leukocytes from the bloodstream into the tissue. The process of diapedesis involves the selective disruption of endothelial cell junctions, which could compromise vascular integrity. It is therefore somewhat surprising that neutrophil transmigration does not significantly impair endothelial barrier function. We examined whether neutrophils might secrete factors that promote vascular integrity during the latter stages of neutrophil transmigration, in particular, the role of neutrophil serine proteinase 3 (PR3).

Methods and results

Endothelial cells were treated with PR3 either in its soluble form or i

SUBMITTER: Kuckleburg CJ 

PROVIDER: S-EPMC3562705 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature