Coiled-coil protein Scy is a key component of a multiprotein assembly controlling polarized growth in Streptomyces.

ABSTRACT: Polarized growth in eukaryotes requires polar multiprotein complexes. Here, we establish that selection and maintenance of cell polarity for growth also requires a dedicated multiprotein assembly in the filamentous bacterium, Streptomyces coelicolor. We present evidence for a tip organizing center and confirm two of its main components: Scy (Streptomyces cytoskeletal element), a unique bacterial coiled-coil protein with an unusual repeat periodicity, and the known polarity determinant DivIVA. We also establish a link between the tip organizing center and the filament-forming protein FilP. Interestingly, both deletion and overproduction of Scy generated multiple polarity centers, suggesting a mechanism wherein Scy can both promote and limit the number of emerging polarity centers via the or

SUBMITTER: Holmes NA 

PROVIDER: S-EPMC3562780 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature