Identification and profiling of conserved and novel microRNAs from Chinese Qinchuan bovine longissimus thoracis.

ABSTRACT:

Background

MicroRNAs (miRNAs) are a family of ~22 nucleotide small RNA molecules that regulate gene expression by fully or partially binding to their complementary sequences. Recently, a large number of miRNAs and their expression patterns have been identified in various species. However, to date no miRNAs have been reported to modulate muscle development in beef cattle.

Results

Total RNAs from the Chinese Qinchuan bovine longissimus thoracis at fetal and adult stages were used to construct small RNA libraries for Solexa SBS technology sequencing. A total of 15,454,182 clean reads were obtained from the fetal bovine library and 13,558,164 clean reads from the adult bovine library. In total, 521 miRNAs including 104 novel miRNA candidates were identified. Furthermore, the nuc

SUBMITTER: Sun J 

PROVIDER: S-EPMC3563516 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature