Transcriptome of the dead: characterisation of immune genes and marker development from necropsy samples in a free-ranging marine mammal.

ABSTRACT:

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Background

Transcriptomes are powerful resources, providing a window on the expressed portion of the genome that can be generated rapidly and at low cost for virtually any organism. However, because many genes have tissue-specific expression patterns, developing a complete transcriptome usually requires a 'discovery pool' of individuals to be sacrificed in order to harvest mRNA from as many different types of tissue as possible. This hinders transcriptome development in large, charismatic and endangered species, many of which stand the most to gain from such approaches. To circumvent this problem in a model pinniped species, we 454 sequenced cDNA from testis, heart, spleen, intestine, kidney and lung tissues obtained from nine adult male Antarctic fur seals (Arct

SUBMITTER: Hoffman JI 

PROVIDER: S-EPMC3563519 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature