Meiotic maps of sockeye salmon derived from massively parallel DNA sequencing.

ABSTRACT:

Background

Meiotic maps are a key tool for comparative genomics and association mapping studies. Next-generation sequencing and genotyping by sequencing are speeding the processes of SNP discovery and the development of new genetic tools, including meiotic maps for numerous species. Currently there are limited genetic resources for sockeye salmon, Oncorhynchus nerka. We develop the first dense meiotic map for sockeye salmon using a combination of novel SNPs found in restriction site associated DNA (RAD tags) and SNPs available from existing expressed sequence tag (EST) based assays.

Results

We discovered and genotyped putative SNPs in 3,430 RAD tags. We removed paralogous sequence variants leaving 1,672 SNPs; these were combined with 53 EST-based SNP genotypes for linkage ma

SUBMITTER: Everett MV 

PROVIDER: S-EPMC3563581 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature