Phylogenetic analysis and DNA-based species confirmation in Anopheles (Nyssorhynchus).

ABSTRACT: Specimens of neotropical Anopheles (Nyssorhynchus) were collected and identified morphologically. We amplified three genes for phylogenetic analysis-the single copy nuclear white and CAD genes, and the COI barcode region. Since we had multiple specimens for most species we were able to test how well the single or combined genes were able to corroborate morphologically defined species by placing the species into exclusive groups. We found that single genes, including the COI barcode region, were poor at confirming species, but that the three genes combined were able to do so much better. This has implications for species identification, species delimitation, and species discovery, and we caution that single genes are not enough. Higher level groupings were partially resolved with some well-

SUBMITTER: Foster PG 

PROVIDER: S-EPMC3563636 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature