Fasciola hepatica and Schistosoma mansoni: identification of common proteins by comparative proteomic analysis.

ABSTRACT: It is not unusual to find common molecules among parasites of different species, genera, or phyla. When those molecules are antigenic, they may be used for developing drugs or vaccines that simultaneously target different species or genera of parasite. In the present study, we used a proteomic-based approach to identify proteins that are common to adult Fasciola hepatica and Schistosoma mansoni. Whole-worm extracts from each parasite were separated by 2-dimensional electrophoresis (2-DE), and digital images of both proteomes were superimposed using imaging software to identify proteins with identical isoelectric points and molecular weights. Protein identities were determined by mass spectrometry. Imaging and immunoblot analyses identified 28 immunoreactive proteins that are common to both

SUBMITTER: Boukli NM 

PROVIDER: S-EPMC3564592 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature