Ontology highlight
ABSTRACT:
SUBMITTER: Hess GT
PROVIDER: S-EPMC3564602 | biostudies-literature | 2012 Jul
REPOSITORIES: biostudies-literature
Bioconjugate chemistry 20120703 7
We exploit bacterial sortases to attach a variety of moieties to the capsid proteins of M13 bacteriophage. We show that pIII, pIX, and pVIII can be functionalized with entities ranging from small molecules (e.g., fluorophores, biotin) to correctly folded proteins (e.g., GFP, antibodies, streptavidin) in a site-specific manner, and with yields that surpass those of any reported using phage display technology. A case in point is modification of pVIII. While a phage vector limits the size of the in ...[more]