Label-free electrochemical detection of an Entamoeba histolytica antigen using cell-free yeast-scFv probes.

ABSTRACT: Inexpensive, simple and quick detection of pathogen antigens in human samples is a key global health objective. Limiting factors include the cost and complexity of diagnostic tests that utilize antibody probes. Herein, we present a method for label-free electrochemical detection of a protein from the enteric pathogen Entamoeba histolytica using cell-free yeast-embedded antibody-like fragments (yeast-scFv) as novel affinity reagents.

SUBMITTER: Grewal YS 

PROVIDER: S-EPMC3564640 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature