Multiplex transcriptional analysis of paraffin-embedded liver needle biopsy from patients with liver fibrosis.

ABSTRACT:

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Background

The possibility of extracting RNA and measuring RNA expression from paraffin sections can allow extensive investigations on stored paraffin samples obtained from diseased livers and could help with studies of the natural history of liver fibrosis and inflammation, and in particular, correlate basic mechanisms to clinical outcomes.

Results

To address this issue, a pilot study of multiplex gene expression using branched-chain DNA technology was conducted to directly measure mRNA expression in formalin-fixed paraffin-embedded needle biopsy samples of human liver. Twenty-five genes were selected for evaluation based on evidence obtained from human fibrotic liver, a rat BDL model and in vitro cultures of immortalized human hepatic stellate cells. The

SUBMITTER: Staten NR 

PROVIDER: S-EPMC3564743 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature