Modulation of glycan detection on specific glycoproteins by lectin multimerization.

ABSTRACT: Improved methods for studying glycans could spur significant advances in the understanding and application of glycobiology. The use of affinity reagents such as lectins and glycan-binding antibodies is a valuable complement to methods involving mass spectrometry and chromatography. Many lectins, however, are not useful as analytic tools due to low affinity in vitro. As an approach to increasing lectin avidity to targeted glycans, we tested the use of lectin multimerization. Several biotinylated lectins were linked together through streptavidin interactions. The binding of certain lectins for purified glycoproteins and glycoproteins captured directly out of biological solutions was increased using multimerization, resulting in the detection of lower concentrations of glycoprotein than possi

SUBMITTER: Cao Z 

PROVIDER: S-EPMC3565077 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature