Classical swine fever virus failed to activate nuclear factor-kappa b signaling pathway both in vitro and in vivo.

ABSTRACT:

Background

Classical swine fever virus (CSFV) is the cause of CSF which is a severe disease of pigs, leading to heavy economic losses in many regions of the world. Nuclear factor-kappa B (NF-κB) is a critical regulator of innate and adaptive immunity, and commonly activated upon viral infection. In our previous study, we found that CSFV could suppress the maturation and modulate the functions of monocyte-derived dendritic cells (Mo-DCs) without activating NF-κB pathway. To further prove the effects of CSFV on the NF-κB signaling pathway, we investigated the activity of NF-κB after CSFV infection in vivo and in vitro.

Methods

Attenuated Thiverval strain and virulent wild-type GXW-07 strain were used as challenge viruses in this study. Porcine kidney 15 (PK-15) cells were cult

SUBMITTER: Chen LJ 

PROVIDER: S-EPMC3565942 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature