The impact of reference gene selection in quantification of gene expression levels in guinea pig cervical tissues and cells.

ABSTRACT:

Background

Accurate measurements of mRNA expression levels in tissues or cells are crucially dependent on the use of relevant reference genes for normalization of data. In this study we used quantitative real-time PCR and two Excel-based applets (geNorm and BestKeeper) to determine the best reference genes for quantification of target gene mRNA in a complex tissue organ such as the guinea pig cervix.

Results

Gene expression studies were conducted in cervical epithelium and stroma during pregnancy and parturition and in cultures of primary cells from this tissue. Among 15 reference gene candidates examined, both geNorm and BestKeeper found CLF1 and CLTC to be the most stable in cervical stroma and cervical epithelium, ACTB and PPIB in primary stroma cells, and CLTC and PPIB i

SUBMITTER: Lindqvist A 

PROVIDER: S-EPMC3565975 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature