Targeted proteomic dissection of Toxoplasma cytoskeleton sub-compartments using MORN1.

ABSTRACT: The basal complex in Toxoplasma functions as the contractile ring in the cell division process. Basal complex contraction tapers the daughter cytoskeleton toward the basal end and is required for daughter segregation. We have previously shown that the protein MORN1 is essential for basal complex assembly and likely acts as a scaffolding protein. To further our understanding of the basal complex, we combined subcellular fractionation with an affinity purification of the MORN1 complex and identified its protein composition. We identified two new components of the basal complex, one of which uniquely associated with the basal complex in mature parasites, the first of its kind. In addition, we identified several other novel cytoskeleton proteins with different spatiotemporal dynamics throughou

SUBMITTER: Lorestani A 

PROVIDER: S-EPMC3566231 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature