Targeted and genome-scale strategies reveal gene-body methylation signatures in human cells.

ABSTRACT: Studies of epigenetic modifications would benefit from improved methods for high-throughput methylation profiling. We introduce two complementary approaches that use next-generation sequencing technology to detect cytosine methylation. In the first method, we designed approximately 10,000 bisulfite padlock probes to profile approximately 7,000 CpG locations distributed over the ENCODE pilot project regions and applied them to human B-lymphocytes, fibroblasts and induced pluripotent stem cells. This unbiased choice of targets takes advantage of existing expression and chromatin immunoprecipitation data and enabled us to observe a pattern of low promoter methylation and high gene-body methylation in highly expressed genes. The second method, methyl-sensitive cut counting, generated nontarget

SUBMITTER: Ball MP 

PROVIDER: S-EPMC3566772 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature