Linking proteomic and transcriptional data through the interactome and epigenome reveals a map of oncogene-induced signaling.

ABSTRACT: Cellular signal transduction generally involves cascades of post-translational protein modifications that rapidly catalyze changes in protein-DNA interactions and gene expression. High-throughput measurements are improving our ability to study each of these stages individually, but do not capture the connections between them. Here we present an approach for building a network of physical links among these data that can be used to prioritize targets for pharmacological intervention. Our method recovers the critical missing links between proteomic and transcriptional data by relating changes in chromatin accessibility to changes in expression and then uses these links to connect proteomic and transcriptome data. We applied our approach to integrate epigenomic, phosphoproteomic and transcript

SUBMITTER: Huang SS 

PROVIDER: S-EPMC3567149 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature