The relationship between long-range chromatin occupancy and polymerization of the Drosophila ETS family transcriptional repressor Yan.

ABSTRACT: ETS family transcription factors are evolutionarily conserved downstream effectors of Ras/MAPK signaling with critical roles in development and cancer. In Drosophila, the ETS repressor Yan regulates cell proliferation and differentiation in a variety of tissues; however, the mechanisms of Yan-mediated repression are not well understood and only a few direct target genes have been identified. Yan, like its human ortholog TEL1, self-associates through an N-terminal sterile α-motif (SAM), leading to speculation that Yan/TEL1 polymers may spread along chromatin to form large repressive domains. To test this hypothesis, we created a monomeric form of Yan by recombineering a point mutation that blocks SAM-mediated self-association into the yan genomic locus and compared its genome-wide chromatin

SUBMITTER: Webber JL 

PROVIDER: S-EPMC3567750 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature