Proteomics and phosphoproteomics analysis of human lens fiber cell membranes.

ABSTRACT:

Purpose

The human lens fiber cell insoluble membrane fraction contains important membrane proteins, cytoskeletal proteins, and cytosolic proteins that are strongly associated with the membrane. The purpose of this study was to characterize the lens fiber cell membrane proteome and phosphoproteome from human lenses.

Methods

HPLC-mass spectrometry-based multidimensional protein identification technology (MudPIT), without or with phosphopeptide enrichment, was applied to study the proteome and phosphoproteome of lens fiber cell membranes, respectively.

Results

In total, 951 proteins were identified, including 379 integral membrane and membrane-associated proteins. Enriched gene categories and pathways based on the proteomic analysis include carbohydrate metabolism (glyco

SUBMITTER: Wang Z 

PROVIDER: S-EPMC3567755 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature