Suitability of endogenous reference genes for gene expression studies with human intraocular endothelial cells.

ABSTRACT:

Background

The use of quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) has become widely applied as a method to measure transcript abundance. In order to be reflective of biological processes during health and disease this method is dependent on normalisation of data against stable endogenous controls. However, these genes can vary in their stability in different cell types. The importance of reference gene validation for a particular cell type is now well recognised and is an important step in any gene expression study.

Results

Cultured primary human choroidal and retinal endothelial cells were treated with the immunostimulant polyinosinic: polycytidylic acid or untreated. qRT-PCR was used to quantify the expression levels of 10 commonly used

SUBMITTER: Wei R 

PROVIDER: S-EPMC3567942 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature