Temperature- and salinity-decoupled overproduction of hydroxyectoine by Chromohalobacter salexigens.

ABSTRACT: Hydroxyectoine overproduction by the natural producer Chromohalobacter salexigens is presented in this study. Genetically engineered strains were constructed that at low salinity coexpressed, in a vector derived from a native plasmid, the ectoine (ectABC) and hydroxyectoine (ectD) genes under the control of the ectA promoter, in a temperature-independent manner. Hydroxyectoine production was further improved by increasing the copies of ectD and using a C. salexigens genetic background unable to synthesize ectoines.

SUBMITTER: Rodriguez-Moya J 

PROVIDER: S-EPMC3568561 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature