Molecular diversity of Bacteroidales in fecal and environmental samples and swine-associated subpopulations.

ABSTRACT: Several swine-specific microbial source tracking methods are based on PCR assays targeting Bacteroidales 16S rRNA gene sequences. The limited application of these assays can be explained by the poor understanding of their molecular diversity in fecal sources and environmental waters. In order to address this, we studied the diversity of 9,340 partial (>600 bp in length) Bacteroidales 16S rRNA gene sequences from 13 fecal sources and nine feces-contaminated watersheds. The compositions of major Bacteroidales populations were analyzed to determine which host and environmental sequences were contributing to each group. This information allowed us to identify populations which were both exclusive to swine fecal sources and detected in swine-contaminated waters. Phylogenetic and diversity analy

SUBMITTER: Lamendella R 

PROVIDER: S-EPMC3568562 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature