Real-time multiplex PCR assay for detection of Brucella spp., B. abortus, and B. melitensis.

ABSTRACT: The identification of Brucella can be a time-consuming and labor-intensive process that places personnel at risk for laboratory-acquired infection. Here, we describe a real-time PCR assay for confirmation of presumptive Brucella isolates. The assay was designed in a multiplex format that will allow the rapid identification of Brucella spp., B. abortus, and B. melitensis in a single test.

SUBMITTER: Probert WS 

PROVIDER: S-EPMC356861 | biostudies-literature | 2004 Mar

REPOSITORIES: biostudies-literature