Interleukin-21 receptor gene polymorphisms in kawasaki disease.

ABSTRACT:

Background and objectives

Interleukin-21 receptor (IL-21R) gene polymorphism is related with the development of systemic vasculitis. In this study, we investigated the polymorphisms of IL-21R gene in patients with Kawasaki disease (KD).

Subjects and methods

We genotyped the promoter region of IL-21R gene (-2500 bp to +1 bp) in 100 patients with KD and 100 healthy controls. All study subjects were Korean. We designed five pairs of primers and performed polymerase chain reaction (PCR) and direct sequencing. We analyzed whole promoter sequences of 200 individuals with comparison to reference sequences of IL-21R gene (NG_012222.1/NC_000016.9).

Results

We found five single nucleotide polymorphisms (SNPs) of which minor allele frequency (MAF) >0.01 in the promoter region of

SUBMITTER: Kim MH 

PROVIDER: S-EPMC3569565 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature