Tomosyn inhibits priming of large dense-core vesicles in a calcium-dependent manner.

ABSTRACT: Neurotransmitter release is a multistep process that is coordinated by a large number of synaptic proteins and depends on proper protein-protein interactions. Using morphological, capacitance, and amperometric measurements, we investigated the effect of tomosyn, a Syntaxin-binding protein, on the different kinetic components of exocytosis in adrenal chromaffin cells. Overexpression of tomosyn decreased the release probability and led to a 50% reduction in the number of fusion-competent vesicles. The number of docked vesicles and the fusion kinetics of single vesicles were not altered suggesting that tomosyn inhibits the priming step. Interestingly, this inhibition is partially relieved at elevated calcium concentration. Calcium ramp experiments supported the latter finding and indicated th

SUBMITTER: Yizhar O 

PROVIDER: S-EPMC356992 | biostudies-literature | 2004 Feb

REPOSITORIES: biostudies-literature