Carbamazepine directly inhibits adipocyte differentiation through activation of the ERK 1/2 pathway.

ABSTRACT:

Background and purpose

Carbamazepine (CBZ), known for its anti-epileptic, analgesic and mood-stabilizing properties, is also known to induce weight gain but the pathophysiology of this adverse effect is still largely unknown. We tested the hypothesis that CBZ could have a direct effect on adipocyte development and metabolism. EXPERIMENTAL RESEARCH: We studied the effects of CBZ on morphological biochemical and molecular markers of adipogenesis, using several pre-adipocyte murine cell lines (3T3-L1, 3T3-F442A and T37i cells) and primary cultures of human pre-adipocytes. To delineate the mechanisms underlying the effect of CBZ, clonal expansion of pre-adipocytes, pro-adipogenic transcription factors, glucose uptake and lipolysis were also examined.

Key results

CBZ strongly inh

SUBMITTER: Turpin E 

PROVIDER: S-EPMC3570010 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature