T cell activation induces proteasomal degradation of Argonaute and rapid remodeling of the microRNA repertoire.

ABSTRACT: Activation induces extensive changes in the gene expression program of naive CD4(+) T cells, promoting their differentiation into helper T cells that coordinate immune responses. MicroRNAs (miRNAs) play a critical role in this process, and miRNA expression also changes dramatically during T cell differentiation. Quantitative analyses revealed that T cell activation induces global posttranscriptional miRNA down-regulation in vitro and in vivo. Argonaute (Ago) proteins, the core effector proteins of the miRNA-induced silencing complex (miRISC), were also posttranscriptionally down-regulated during T cell activation. Ago2 was inducibly ubiquitinated in activated T cells and its down-regulation was inhibited by the proteasome inhibitor MG132. Therefore, activation-induced miRNA down-regulation

SUBMITTER: Bronevetsky Y 

PROVIDER: S-EPMC3570096 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature