Molecular profiling of cutaneous squamous cell carcinomas and actinic keratoses from organ transplant recipients.

ABSTRACT:

Background

The risk of developing cutaneous squamous cell carcinoma (SCC) is markedly increased in organ transplant recipients (OTRs) compared to the normal population. Next to sun exposure, the immunosuppressive regimen is an important risk factor for the development of SCC in OTRs. Various gene mutations (e.g. TP53) and genetic alterations (e.g. loss of CDKN2A, amplification of RAS) have been found in SCCs. The aim of this genome-wide study was to identify pathways and genomic alterations that are consistently involved in the formation of SCCs and their precursor lesions, actinic keratoses (AKs).

Methods

To perform the analysis in an isogenic background, RNA and DNA were isolated from SCC, AK and normal (unexposed) epidermis (NS) from each of 13 OTRs. Samples were subjecte

SUBMITTER: Hameetman L 

PROVIDER: S-EPMC3570297 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature