Vasohibin-1 is identified as a master-regulator of endothelial cell apoptosis using gene network analysis.

ABSTRACT:

Background

Apoptosis is a critical process in endothelial cell (EC) biology and pathology, which has been extensively studied at protein level. Numerous gene expression studies of EC apoptosis have also been performed, however few attempts have been made to use gene expression data to identify the molecular relationships and master regulators that underlie EC apoptosis. Therefore, we sought to understand these relationships by generating a Bayesian gene regulatory network (GRN) model.

Results

ECs were induced to undergo apoptosis using serum withdrawal and followed over a time course in triplicate, using microarrays. When generating the GRN, this EC time course data was supplemented by a library of microarray data from EC treated with siRNAs targeting over 350 signalling mol

SUBMITTER: Affara M 

PROVIDER: S-EPMC3570387 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature