Ontology highlight
ABSTRACT:
SUBMITTER: Mashimo T
PROVIDER: S-EPMC3570776 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Mashimo Tomoji T Kaneko Takehito T Sakuma Tetsushi T Kobayashi Junya J Kunihiro Yayoi Y Voigt Birger B Yamamoto Takashi T Serikawa Tadao T
Scientific reports 20130213
TAL Effector Nucleases (TALENs) are versatile tools for targeted gene editing in various species. However, their efficiency is still insufficient, especially in mammalian embryos. Here, we showed that combined expression of Exonuclease 1 (Exo1) with engineered site-specific TALENs provided highly efficient disruption of the endogenous gene in rat fibroblast cells. A similar increased efficiency of up to ~30% with Exo1 was also observed in fertilized rat eggs, and in the production of knockout ra ...[more]