Identification of the dimer interface of a bacterial Ca(2+)/H(+) antiporter.

ABSTRACT: Members of the calcium/cation antiporter superfamily, including the cardiac sodium/calcium exchangers, are secondary active transporters that play an essential role in cellular Ca(2+) homeostasis. A notable feature of this group of transporters is the high levels of sequence similarity in relatively short sequences constituting the functionally important α-1 and α-2 regions in contrast to relatively lower degrees of similarity in the extended adjoining sequences. This suggests a similar structure and function of core transport machinery but possible differences in topology and/or oligomerization, a topic that has not been adequately addressed. Here we present the first example of purification of a bacterial member of this superfamily (CAX(CK31)) and analyze its quaternary structure. Purifi

SUBMITTER: Ridilla M 

PROVIDER: S-EPMC3571656 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature