Rapid focused sequencing: a multiplexed assay for simultaneous detection and strain typing of Bacillus anthracis, Francisella tularensis, and Yersinia pestis.

ABSTRACT:

Background

The intentional release of Bacillus anthracis in the United States in 2001 has heightened concern about the use of pathogenic microorganisms in bioterrorism attacks. Many of the deadliest bacteria, including the Class A Select Agents Bacillus anthracis, Francisella tularensis, and Yersinia pestis, are highly infectious via the pulmonary route when released in aerosolized form. Hence, rapid, sensitive, and reliable methods for detection of these biothreats and characterization of their potential impact on the exposed population are of critical importance to initiate and support rapid military, public health, and clinical responses.

Methodology/principal findings

We have developed microfluidic multiplexed PCR and sequencing assays based on the simultaneous interroga

SUBMITTER: Turingan RS 

PROVIDER: S-EPMC3572037 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature