Shotgun proteomic analysis of plasma from dairy cattle suffering from footrot: characterization of potential disease-associated factors.

ABSTRACT: The plasma proteome of healthy dairy cattle and those with footrot was investigated using a shotgun LC-MS/MS approach. In total, 648 proteins were identified in healthy plasma samples, of which 234 were non-redundant proteins and 123 were high-confidence proteins; 712 proteins were identified from footrot plasma samples, of which 272 were non-redundant proteins and 138 were high-confidence proteins. The high-confidence proteins showed significant differences between healthy and footrot plasma samples in molecular weight, isoelectric points and the Gene Ontology categories. 22 proteins were found that may differentiate between the two sets of plasma proteins, of which 16 potential differential expression (PDE) proteins from footrot plasma involved in immunoglobulins, innate immune recogniti

SUBMITTER: Sun D 

PROVIDER: S-EPMC3572155 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature