Association between DNA methylation of HSPB1 and death in low Gleason score prostate cancer.

ABSTRACT:

Background

Heat shock protein 27 (Hsp-27) encoded by gene HSPB1 is a critical regulator of the behavioral phenotype of human prostate cancer (PCa) cells, enhanced expression being associated with highly aggressive disease and poor clinical outcome. In contrast, the protein is not expressed in PCas of low malignant potential. To gain insight into the mechanism regulating its expression, we tested the hypothesis that differential methylation of CpG islands within HSPB1 controls transcription and subsequent translation of the gene.

Methods

We studied prostate epithelial cell lines and tissue biopsies, including 59 BPH and 415 PCas, of which 367 were a cohort of men with up to 20 years of follow-up. Methylation across the gene (DNA methylation (DNAme)) was assayed by pyrosequenc

SUBMITTER: Vasiljevic N 

PROVIDER: S-EPMC3572391 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature