Use of recombinant purified protein derivative (PPD) antigens as specific skin test for tuberculosis.

ABSTRACT:

Background & objectives

Purified protein derivative (PPD) is currently the only available skin test reagent used worldwide for the diagnosis of tuberculosis (TB). The aim of this study was to develop a Mycobacterium tuberculosis specific skin test reagent, without false positive results due to Bacillus Calmette-Guerin (BCG) vaccination using recombinant antigens.

Methods

Proteins in PPD IC-65 were analyzed by tandem mass spectrometry and compared to proteins in M. tuberculosis culture filtrate; 54 proteins were found in common. Top candidates MPT64, ESAT 6, and CFP 10 were overexpressed in Escherichia coli expression strains and purified as recombinant proteins. To formulate optimal immunodiagnostic PPD cocktails, the antigens were evaluated by skin testing guinea pigs sensi

SUBMITTER: Stavri H 

PROVIDER: S-EPMC3573601 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature