Evidence for an ABC-type riboflavin transporter system in pathogenic spirochetes.

ABSTRACT:

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Bacterial transporter proteins are involved in the translocation of many essential nutrients and metabolites. However, many of these key bacterial transport systems remain to be identified, including those involved in the transport of riboflavin (vitamin B(2)). Pathogenic spirochetes lack riboflavin biosynthetic pathways, implying reliance on obtaining riboflavin from their hosts. Using structural and functional characterizations of possible ligand-binding components, we have identified an ABC-type riboflavin transport system within pathogenic spirochetes. The putative lipoprotein ligand-binding components of these systems from three different spirochetes were cloned, hyperexpressed in Escherichia coli, and purified to homogeneity. Solutions of all three of the purified

SUBMITTER: Deka RK 

PROVIDER: S-EPMC3573665 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature