Molecular imaging of the paracrine proangiogenic effects of progenitor cell therapy in limb ischemia.

ABSTRACT:

Background

Stem cells are thought to enhance vascular remodeling in ischemic tissue in part through paracrine effects. Using molecular imaging, we tested the hypothesis that treatment of limb ischemia with multipotential adult progenitor cells (MAPCs) promotes recovery of blood flow through the recruitment of proangiogenic monocytes.

Methods and results

Hind-limb ischemia was produced in mice by iliac artery ligation, and MAPCs were administered intramuscularly on day 1. Optical imaging of luciferase-transfected MAPCs indicated that cells survived for 1 week. Contrast-enhanced ultrasound on days 3, 7, and 21 showed a more complete recovery of blood flow and greater expansion of microvascular blood volume in MAPC-treated mice than in controls. Fluorescent microangiography dem

SUBMITTER: Ryu JC 

PROVIDER: S-EPMC3573704 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature