Transferring a synthetic gene circuit from yeast to mammalian cells.

ABSTRACT: The emerging field of synthetic biology builds gene circuits for scientific, industrial and therapeutic needs. Adaptability of synthetic gene circuits across different organisms could enable a synthetic biology pipeline, where circuits are designed in silico, characterized in microbes and reimplemented in mammalian settings for practical usage. However, the processes affecting gene circuit adaptability have not been systematically investigated. Here we construct a mammalian version of a negative feedback-based 'linearizer' gene circuit previously developed in yeast. The first naïve mammalian prototype was non-functional, but a computational model suggested that we could recover function by improving gene expression and protein localization. After rationally developing and combining new par

SUBMITTER: Nevozhay D 

PROVIDER: S-EPMC3573884 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature