Saturated linkage map construction in Rubus idaeus using genotyping by sequencing and genome-independent imputation.

ABSTRACT:

Background

Rapid development of highly saturated genetic maps aids molecular breeding, which can accelerate gain per breeding cycle in woody perennial plants such as Rubus idaeus (red raspberry). Recently, robust genotyping methods based on high-throughput sequencing were developed, which provide high marker density, but result in some genotype errors and a large number of missing genotype values. Imputation can reduce the number of missing values and can correct genotyping errors, but current methods of imputation require a reference genome and thus are not an option for most species.

Results

Genotyping by Sequencing (GBS) was used to produce highly saturated maps for a R. idaeus pseudo-testcross progeny. While low coverage and high variance in sequencing resulted in a larg

SUBMITTER: Ward JA 

PROVIDER: S-EPMC3575332 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature