Integrative analyses identify osteopontin, LAMB3 and ITGB1 as critical pro-metastatic genes for lung cancer.

ABSTRACT:

Objective

To explore the key regulatory genes associated with lung cancer in order to reduce its occurrence and progress through silencing these key genes.

Methods

To identify the key regulatory genes involved in lung cancer, we performed a combination of gene array and bioinformatics analyses to compare gene transcription profiles in 3 monoclonal cell strains with high, medium or low metastatic abilities, which were separated from the SPC-A-1sci and SPC-A-1 cell lines by limiting dilution monoclone assay. We then analyzed those genes' biological activities by knocking down their expression in SPC-A-1sci cells using siRNA and lenti-viral shRNA vectors, followed by determinations of the invasion and migration capabilities of the resulting cell lines in vitro as well as their

SUBMITTER: Wang XM 

PROVIDER: S-EPMC3575388 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature